What Constitutes Good CRISPR Libraries?

Date: July 27, 2017

Time: 11:00 am EST, 8 am PST, 5 pm Paris

Speaker: Peter Sheffield, Ph.D., R&D Scientific Program Director

Attendees of this 60-minute webinar will gain an insight into the underlying principles of how to design an in silico pooled CRISPR library. Having an in silico CRISPR library design is the first step in planning and executing a successful CRISPR genome editing experiment. Converting a CRISPR design into a functional CRISPR reagent is a multistep process wherein each step has the potential to introduce variation into the library which in turn can lead to poor experimental outcomes. Agilent experts will discuss guidelines and techniques to help create a high quality CRISPR library to ensure the best possible outcome when performing genome editing experiments.

Topics that will be covered in this webinar include:

  • Principles of CRISPR pool library design
  • How to create a high quality cloned lentiviral plasmid CRISPR library
  • Which library parameters help you assess the quality of your cloned library
  • Delivery of your CRISPR and Cas9 reagents into your cell line
  • Next Generation sequencing principles behind CRISPR Library analysis

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Trusted Answers. Together.

For Research Use Only. Not for use in diagnostic procedures.

 

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